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This short scene is between Konata and Tsukasa, some time after the previous scene, still in school.
(Tsukasa: Kona-chan, muzukashii kao shite dou shita no?)
こなちゃん (Kona-chan): is Konata’s nickname. Japanese nicknames often use only 2 morae, so Konata gets shortened to Kona. The “chan” address suffix is used between intimate girls who are intimate friends and grown-ups towards children and and people towards their pets. If you’re aspiring to be chatty, don’t try to call a girl “chan.” You might get in trouble.
難しい (muzukashii): is an adjective conjugated for the affirmative, present meaning “hard” or “serious.”
顔 (kao): is a nou meaning “face.”
して (shite): is the Te-form of the verb “suru,” meaning “to do.” It’s part of a construction. “Kao suru” is “to make a face,” by the way.
どうしたの (dou shita no): is an expression meaning “what’s up with X?” (or simply “why”?) where X is the verb phrase conjugated as the Te-form. “Dou” is an adverb meaning “how?” “Shita” is the affirmative, past conjugation of “suru.” And “no” is the substantivizing suffix.
Translation: “Konata, why are you making that serious face?”
(Konata: Iya, taishita koto ja nai kedo kesa no kaiwa de ki ni naru koto ga.)
いや (iya): is an interjection meaning “No.” It comes from the noun “iya,” meaning “disagreeable.”
大した (taishita): is a really interesting word. It’s syntactically a verb that doesn’t seem to conjugate as one would expect. (That “shita” is the past conjugation of “suru;” but there’s not “taisuru.” It means “big” or “important.”
事 (koto): is the same “koto” as before, now you can see the Kanji.
じゃ (ja): is a contraction of “de wa,” which is equivalent to the topical particle “wa.”
ない (nai): is the same as before. (See Part 19)
けど (kedo): is a conjunction meaning “though.” “XけどY” means “Though X, Y.”
今朝 (kesa): is a noun meaning “morning.”
の (no): is our genitive particle.
会話 (kaiwa): is a noun meaning “conversation.”
で (de): is the instrumental particle indicating cause. (See Part 19)
気 (ki): is a noun meaning “spirit” or “energy.” This is part of an expression.
に (ni): is the adverbial suffix. (I’m changing my mind about “ni naru,” I am aware.)
なる (naru): is the same verb as before. (See Part 19) “Ki ni naru” means “to have on one’s mind.” or more literally “to become energetic…”
こと (koto): is the same as before.
が (ga): is the conjunction we saw before. (See Part 19)
Translation: “No, though it’s not important, it’s been on my mind because of this morning’s conversation.”
(Tsukasa: E? Kaze to infuruenza no koto?)
Everything here has been covered before. (See Part 19)
But I’ll note that “no” is the genitive particle; but I’m translating it as “about” because that’s what it would be in English.
Translation: “What? The thing about the cold and influenza?”
(Konata: Tsukasa, watashi ga baka tte bubun hitei shinakatta naa tte.)
私 (watashi): is the first person singular pronoun. “I”
が (ga): is the nominative particle.
バカ (baka): is a noun meaning “idiot.”
って (tte): is a casual topical particle. So its functionally equivalent to “wa.”
部分 (bubun): is the same as before. (See Part 19)
否定 (hitei): is a noun meaning “negation.” A “bubun hitei” is a “partial negation” or a “negation in part.”
しなかった (shinakatta): is the negative, past conjugation of “suru.” Nouns plus “suru” are verbalized semantically. So if you can make the noun more verb-y somehow, your translation will be just fine.
なぁ (naa): is a contemplative ending particle. One can translate it as “huh…”
って (tte): is also a casual quotative particle. Konata is letting Tsukasa know what she was thinking about. So the omitted verb is “omou,” meaning “to think.”
Translation: “About me being an idiot, you didn’t partially deny it…”
(Tsukasa: Ch-chigau. souiu-n ja nakute…)
違がう (chigau): is the same as before. One can make a case for the translation to be “wrong” or “different,” the important thing being that it’s clear that what Konata is implying is not true.
そういう (souiu): is a adjectival expression meaning “like that.” It comes from “sou,” like “sou desu ka,” the adverb meaning “so” and “iu,” the verb meaning “to say.”
ん (n): is equivalent to “no” in that it is a substantivizing suffix.
じゃ (ja): is the same as before.
なくて (nakute): is the Te-form of the negative, present conjugation of “aru,” which we’ve spoken of before.
Translation: “[That’s] wrong. [It] isn’t like that…”
Words Worth Memorizing
難しい (muzukashii): hard; serious
顔 (kao): face
どう (dou): how?
いや (iya): No (interjection)
大した (taishita): important, big
今朝 (kesa): this morning
会話 (kaiwa): conversation
気になる (ki ni naru): to be on one’s mind
私 (watashi): I
バカ (baka): idiot
否定 (hitei): negation
そういう (souiu): like that (expression)